Creating a GPC calibration

To be able to create a GPC calibration, you need to have a measured and integrated GPC chromatogram of a standard sample, and the GPC mode toggled ON by selecting the Setting - GPC Mode in the Instrument window (this is the default setting of GPC instrument).

  1. Open the Calibration window: select Window - GPC Calibration in the Instrument window or click on .
  2. Create a new calibration file: select File - New or click on ⓐ.
  3. Following dialog GPC Calibration Options will show up. Here you can setup various options and Calibration Types.

Caution:

Once selected, the Calibration Type can't be changed later.

  1. Click OK to create the Noname calibration. To set calibration name open the File - Save As dialog.
  2. Open integrated chromatogram of a standard: select File - Open Standard… or click on ⓑ icon.
  3. Add peaks present in the chromatogram of the calibration standard to the calibration file: select Calibration - Add All or click on ⓒ (if you have multiple peaks in your chromatogram) or select Calibration - Add Narrow Peak or click on ⓓ to add desired peak from your standard.

Note:

If you have multiple chromatograms of standards, repeat these steps to add the desired peaks: open the standard and click Add Narrow Peak, then open another standard and click Add Narrow Peak again, repeat it for all standards. The number in field ⓔ is connected to the number of used standard, and not to the concentration level. Adding a peak on an already used number will overwrite values for this number.

Note:

When using any type of Broad calibration, Add Broad Peak will be enabled instead of Add Narrow Peak.

  1. Fill in the appropriate molecular weight values for the respected peaks into the M column ⓕ.
  2. Save the calibration file: from File - Save or click on ⓖ.