How to set PDA method
This section deals only with method setup related only to PDA options.
- The PDA Method tab ① is available only when the PDA Extension is configured on the given instrument.
- From the drop down menu ②, select PDA Spectrum if the chromatogram contains more than one (typically DAD and FLD).
- Peak Purity Options: there are several settings that can restrict the peak purity evaluation. The Restrict Wavelength Range ③ can be used to restrict the peak purity evaluation wavelength range. Use the checkbox and fill the wavelength range.
- Another restriction can be applied using the Absorbance Threshold ④. This determines which part of the peak will be evaluated. Threshold of 0% indicates that the entire peak will be included in calculation. At 5%, the spectra that have less than 5% of the apex spectrum absorbance will be excluded from the peak purity calculation, etc.
- Used Points ⑤ specifies the number of points for the evaluation of peak purity; All indicates all spectral points, while Five uses only the five most significant points (peak start, peak end, peak apex, and two peak purity inflex points).
- In the Library Search Options⑥, select the criteria according to which the Perform Library Search command will be performed (as described in the chapter "Spectrum").
- To add another PDA library to search in, click the
icon ⑦ and then
. Choose the library in the Select Spectral Library dialog.
- The list of libraries that will be searched is listed below ⑧. You can enable/disable the search in specific libraries using the accompanying checkboxes.