Separation

All interval operation follows the rule that with Time B = 0 operation will be applied from Time A to the end of the chromatogram. With both Time A and Time B set to 0, operation is applied over the whole chromatogram.

Valley To Valley Slope

Specifies the maximum slope of the baseline. If the baseline slope is smaller than the specified value, the separating perpendicular line is canceled and the baseline passes through the valley. The default value of 0 prevents the baseline from passing through the valley.

Tangent Area Ratio

Constitutes the first condition imposed on tangential separation. The condition for tangential separation is satisfied if the ratio of areas of the separated and the main peak is lower than specified value. First raider peak not fulfilling the preset conditions ends tangential separation, peaks further from the main peak apex are then integrated to the baseline.

The value of 0 prevents tangential separation. If this operation is selected in the Integration table, its value is set to value of 10 and in general increasing value causes higher degree of tangential separation of the raider peaks. If there is no Tangent Slope Ratio item defined in the Integration table but Tangent Area Ratio is defined, the value of the Tangent Slope Ratio is considered to be 10.

Tangent Slope Ratio

This is the second condition imposed on tangential separation. The condition is satisfied if the angle between the baseline of the raider peak and the baseline of the original cluster peak (connections between starting and final points of the peaks are considered as baselines) exceeds the specified value. The ratio is defined as the tangent to the baseline of the original cluster peak compared to the baseline of the raider. First raider peak not fulfilling the preset conditions ends tangential separation, peaks further from the main peak apex are then integrated to the baseline.

Value of 0 prevents tangential separation. If this operation is selected in the Integration table, its value is set to value of 10 and in general increasing value causes lower degree of tangential separation of the raider peaks. The range of possible values is between 0 - 100, value 0 corresponds to angle 0° and value of 100 corresponds to angle 180°. If there is no Tangent Area Ratio item defined in the Integration table but Tangent Slope Ratio is defined, but the value of the Tangent Area Ratio is considered to be 10.