No identified peak(s)
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Based on the linked calibration it was not possible to identify any peak. Possible reasons are for example incorrect linked calibration or compounds in calibration are not checked as used.
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No ISTD peak detected
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The calculation cannot be performed using the internal standard because the calibration does not contain any peak which has been indicated as internal standard (ISTD). Uncalibrated calculation will be used instead. Make sure ISTD Peak with appropriate Retention Time is entered in the calibration or change the chromatogram Calculation type so the ISTD compound is not requested.
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No valid level in ISTD%d compound
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ISTD peak is identified in the calibration but does not have a valid level. View the linked Calibration, switch to the ISTD compound tab and make sure there is valid level and it is checked as used.
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Missing ISTD Amount in Sample
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When ISTD calculation is selected, it is necessary to enter the ISTD Amount used in the sample. It can be entered in the Common for All Signals part of Results (Chromatogram window). For the ISTD2 - ISTD10 click the button.
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No ISTD Amount given in Calibration
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When ISTD calculation is selected, it is necessary to enter the Calibration ISTD Amount. It can be entered in the Amount column in the Calibration window.
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ISTD (Compound X) is not enabled in the Calibration
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At least one peak in ISTD calculation mode is using ISTD peak which is disabled in the used calibration. The remedy is to select a different ISTD peak or enable the disabled ISTD peak in calibration, which may in turn produce other messages as the disabled ISTD peak was probably not recalibrated. |
Definition of ISTD compund for X peak is not valid. |
In the mode allowing to use the ISTD peak from different signal, a peak from signal not present in the chromatogram should be used as ISTD (e.g. ISTD peak should be set on signal 3, but chromatogram has just 1 signal). |
Peak(s) ISTDX on signal Y not detected.
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One or more of the ISTD peaks defined is not found in it's identification window. |
Chromatogram does not have enough signals - ISTD peak for compound X is not valid.
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Calibration file is used in a mode where ISTD peak can be defined on a different signal. Calibration itself has enough signals so that all ISTD peaks are properly defined, but opened chromatogram has fewer signals and at least one of the cross-signal ISTDs is looked for on non-existing chromatogram signal. |
Some referred ISTD compound not used.
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Only possible in version of integration algorithm older than version Wave rev. 4 or Legacy (10.0 rev . 2). One or more of the ISTD peaks has the Used checkbox in the calibration disabled. |
Calibration curve for X compound could not be constructed
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If calibration curve could not be constructed for one compound in calibration, no results are calculated for any compound. The problematic compound is listed in the error message and can be detected in the Peak Type column as "Error". To remedy, view the linked Calibration and check the Amounts and Responses for given compound are set correctly.
If compounds do not need to be quantified (e.g. Calibration is used only to identify peaks) Free Calibration can be used (On the given compound tab in the Calibration set the Curve Fit Type to Free Calibration for the compound)
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Calibration curve could not be constructed for multiple compounds
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Same as the error message above, only calibration curve could not be constructed for more that one compound. Curves for all compounds have to be constructed to get calculated results. |
Signal is moved/scaled
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It is not possible to quantify results as the signal has been moved or scaled. To get the signal into the original position go to the menu Display - Properties - Signals tab, use the Original button in the Offset & Scale part.
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Not all sample peaks are identified
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This message appears when the Calculation is set to NORM and it is not possible to identify all peaks. It is possible that some required compounds in the calibration have incorrect retention time, are not checked as used or are not entered in the calibration.
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Sample Amount must be zero.
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When the Calculation is set to NORM the Amount must be zero. Both Calculation and Amount can be modified in the Common for All Signals pane in Chromatogram window.
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