This topic describes how to use Clarity, in order to obtain correct results calculated according to normalization calibration method. The normalization can be achieved by two means. First option is applicable to known response factors specific for each analyte. Second option is to use calibration based on standard sample with known fraction composition. In the latter case, Clarity automatically calculates response factors for all analytes that are subsequently applied in evaluation of the unknown sample. The results are displayed in Amount% column in the Summary Table and represent percent fraction of each analyte present in the unknown sample.
For both options set the calibration to the NORM Display mode, this ensures that Clarity checks that all the peaks are used for calculations and 100% Amount really corresponds to the whole sample.
- Set Display Mode to NORM ⓐ in Calibration Options dialog.
A) Application of specific Response Factors
- Use procedure explained in the chapter Calibrating with manually entered Response Factors.
Figure below displays Result Table of a chromatogram with linked calibration that was created using the above mentioned method. Note different values in columns Area [%] and Amount% are caused by various values in Manual Response Factor column.
B) Application of automatically calculated Response Factors based on calibration with standard sample with known composition
- Use procedure explained in the chapter Creating a new calibration.
Figure below displays Result Table of a chromatogram with linked calibration that was created using the above mentioned method. Note different values in columns Area [%] and Amount% are caused by calculated values in Response Factor column.
