Modifying Global parameters

Peaks are only integrated if they exceed a minimum width (Global Peak Width) and height (Global Threshold). The Global Baseline Slope parameter influences the position of peak starts and ends. These parameters can be applied both globally or locally in a specified interval. It is always recommended to maximally utilize global parameters before using any local ones. Global Filter - Bunching works as a smoothing filter, it can to be applied only globally (to the whole chromatogram).

Global Peak Width:

• To adjust the minimum peak width use Chromatogram - Global Peak Width or the icon ⓐ and select the narrowest peak you want to integrate.

Global Threshold:

• To adjust the minimum peak height use Chromatogram - Global Threshold or the icon ⓐ and select an interval containing only noise, not peaks.

Global Filter - Bunching:

• Works as a smoothing filter that automatically averages number of data points in order to preserve at least 30 data points per narrowest peak. It is dependent on the Global Peak Width value. To use it, use Chromatogram - Global Filter - Bunching or the icon ⓐ after you are done adjusting the Global Peak Width parameter.

Global Baseline Slope:

• To adjust the minimum slope where the peak should start/end, use Chromatogram - Global Baseline Slope or the icon ⓐ and select the point where the peak should start/end.

If you are still not happy with the integration suggested by selection from the graph, you can manually modify the parameter values in the Integration Tableⓑ.

Setting the Local variants of Global parameters:

1. Setting local parameters is similar to the global ones, the only difference is that they are only applied on the selected time interval rather than the entire chromatogram.
2. To set a local parameter, use the Chromatogram - Integration context menu and select the parameter you need. Similarly, you can find the icon in the bottom part of the vertical toolbar ⓒ. Select the desired time interval in the graph. A small dialog appears ⓓ, where you can further specify the time interval and mainly select the value of the parameter. You can use the Suggest Value button ⓔ to select the value from the graph same as for a global parameter.
3. Again, all values can be further modified in the Integration Table if needed.