- Open the Sequence window by selecting Analysis - Sequence or click on the
icon in the Instrument window. - Create a new sequence file by click
ⓐ. or open an already created sequence ⓑ. If you already have a sequence ready skip to step 11. - Save the new sequence by selecting File - Save As… and give it a name in the saving dialog.
- Check the checkbox in the first (empty) row in the Run column ⓒ. The row will be pre-filled with default basic information on the analysis.
- Fill in the Sample ID and/or Sample columns ⓓ.
- Fill in the File Name ⓔ. It is recommended to use variables to do so, for example, %q ( Sample ID) and %n (counter) or %R (date and time) to prevent file name conflicts. Hold the mouse pointer over the file name field to see resulting name. For more info regarding variables see "Chromatogram File Name" in the Clarity
- The SV (Starting Vial) and EV (End Vial) rows ⓕ will be pre-filled with numbers corresponding to the sample position in the autosampler tray, if you are using an autosampler. It is possible to change these as needed, injection from multiple vials can be made on one sequence row.
- Fill in volume of the injection in the Inj. Vol. column ⓖ.
- Select the method to be used in the Method Name column.
- Tick any of the Open, Open Calib., Print etc., columns ⓗ in case you wish to open measured chromatogram in chromatogram or calibration window or print the results after each measurement of a sample.
- Repeat the steps 4-10 for rows you need to add to the Sequence Table.
- Check the validity of the sequence by selecting Sequence - Check Sequence or clicking on
icon. In valid sequences, all rows will show the 
symbol in the Status column. Invalid sequences will issue a warning message with the cause of the problem. - Save the sequence by selecting File - Save or clicking on
icon. - Run the prepared sequence by selecting Sequence - Run or clicking on
icon ⓘ.
Note:
The sequence state will change to Waiting for injection or Injecting state, depending on Ready state of other controlled modules. The state is visible at the bottom of the Sequence window.
Caution:
To correctly include chromatograph graphs in the reports it is necessary to check both Open and Print checkboxes. It is also necessary to fill in Report Style when Print or Print to PDF is used.
Note:
It is possible to display additional columns by right-clicking the sequence table, selecting Setup Columns and moving items from Hide Columns to Show Columns list by Show button.
