Option Clone on first recalibration sets the sequence and calibration to the Safe Calibration Usage mode. This option will create a clone (copy) of calibration defined in the method upon completing the first row of the sequence. Cloned calibration is attached to each new chromatogram produced by the given sequence.
- Open the Method Setup dialog - Calculation tab. Select Method - Calculation
from the Instrument window.
- Click on Set... button and select a calibration file ⓐ to be used during cloning at first recalibration.
Note:
This calibration will remain unchanged as newly created clone of the calibration will be used with new responses.
- Create a custom name for the calibration files in Calibration Cloning in Sequence ⓑ as explained in Creating customized file names automatically.
Note:
The name of the final calibration file will match just the content of this field - if you wish to include the name of the template calibration, include the name in this field again (e.g. "test - %s %L %R").
- Follow the steps in Creating and running a sequence to create your sequence based on the example below.
More- Set the row/s for the standard/s at the beginning. ⓒ
- Add a row for a blank, if you wish to.
- Set the row/s for the unknown samples.
- Click
to open the Sequence Options Dialog ⓓ. - Select Clone on first recalibration ⓔ and click OK.
- Run the sequence as explained in Creating and running a sequence.
Note:
The measured sequence can be reprocessed using Batch. For more info see the topic Reprocessing whole sequence while using calibration cloning.