Online - Sequence (or Single Analysis)

During an online validation, the measured chromatograms will be gradually and automatically opened in the Chromatogram window. Validation results will automatically be updated with every new chromatogram.

• In the Chromatogram window switch to the SST Results tab and open the SST method using the SST - Open... command. If no SST method is prepared, create a new one (for more details see also chapter Creating SST method).
• Close any chromatograms that are still open in the Chromatogram window using the File - Close All command.
• Check that the Overlay mode has been enabled. E.g. in the bottom-right corner of the Chromatogram window. If Overlay mode has been disabled (the word “Overlay” is shaded gray), enable it by double-clicking on it or using the File - Overlay Mode command or the icon.
• Ensure that automatic loading of measured chromatograms into the Chromatogram window has been enabled; select the Open in Chromatogram Window checkbox in the Single Analysis - Post-run Options tab or checkbox in the Open column in the Sequence window.
• Switch to the Sequence window and run the prepared sequence. When running a single analyses, gradually run them in the Single Analysis dialog.
• All measured chromatograms will be automatically opened in the Chromatogram window and added to the validation.
• After measuring the final sample, switch to the Chromatogram window, then switch to the SST Results tab and check the validation results.