Sequence
This Chapter describes window which is used for consecutive measurement of multiple samples. Open the window using the 
icon or the Analysis - Sequence command from the
The window includes a toolbar, sequence table and status bar.
Table description
Each line defines the settings for one or more injections. To process several vials from a single line enter a different value for the initial = start (SV) and last = end (EV) vial. The I/V parameter indicates that there will be multiple injections taken from a single vial.
Status of the row in the sequence.
Indicates a row with one or more errors, such row is disabled from running.
Indicates a row with one or more warnings, measurement will still be carried out.
Note:
Warning Calibration already exists is caused by non-unique name of cloned calibration. Adjusting the name for cloned calibration in Method Setup - Calculation resolves the problem.
Point mouse cursor over the icon in the Status column to display a tooltip with detailed description about the injections - it displays how many were measured, skipped, aborted and how many are remaining on the specific row.
Note:
Analysis will be marked as finished after the analysis time elapsed. Note that the instrument method may be still running.
List of possible states of sequence rows
| Status | State | Description |
|---|---|---|
|
Ready | No analysis has been carried out from this row. Measurement will be carried out. |
|
Ready | No analysis has been carried out from this row. At least one vial has been skipped. Rest of the vials will be measured. |
|
Ready | Analysis has been stopped during acquisition. At least one chromatogram has been measured. Rest of the vials will be measured. |
|
Running | Analysis from this row is being carried out. |
|
Completed | All analyses on this row have been measured. |
|
Partially completed | Apart from the vial(s) where the Skip Vial command has been used, all analyses on this row have been measured. |
|
Partially completed | Command Skip Vial has been used on all vials. No more measurement will be carried out from this row. If icon changes color to green and back to original when (un)checking Run checkbox then there are vial(s) that still can be measured. |
|
Partially completed | Analysis has been stopped during acquisition. At least one chromatogram has been measured. Run checkbox is unckecked and measurement will not continue unless checking the Run checkbox. |
|
Disabled | Row that has been omitted from the sequence. Caused either by an error on the row or by unchecked Run checkbox. |
Already measured rows will be blocked against changes (dimmed) in all columns except for Run, Sample Type, Lvl, Report Style and Postrun functions, columns which can be changed for reprocessing.
If there is a chromatogram resulting from this row, small triangle will appear in the icon - e.g. 
(currently measured row with at least one already measured chromatogram). Left mouse click on the triangle will reveal option to open the chromatogram(s). See Fig "Open chromatograms in overlay".
It is possible to open one specific chromatogram or open all measured chromatograms in overlay.
Selected row(s) will return to their initial Status (all fields green) in the following cases:
- Running of the sequence (by Run command, the
icon or F5 or Ctrl + Q shortcut) and clicking Reset button in the displayed dialog. - Using the Edit - Reset Status command (Ctrl + E).
Note:
A text description will be printed in the printouts instead of colored tags in the Status column.
Selects whether the particular line in the Sequence Table will be measured. The column corresponds to the Run Lines field in Sequence Options dialog. Working with Run column is more suitable for discontinuous selection of lines or for exclusion of individual lines from measurement.
Number of the first vial used in the processing of the given row.
Number of the last vial used in the processing of the given row. Its number must be the same or higher than the number entered in the SV field. The difference between the end and start vial defines how many vials will be processed by selected line.
If Options - Format - Automatically setting has been applied, then for more than one vial or injection, the File Name pattern will automatically be verified and corrected to ensure that it is in the correct format. In other words, %v and %i variables will be added to the pattern.
If automatic formatting has been applied, the variables in the filename will be supplemented with zeros from the left in order to correspond with defined number of injected samples (e.g. names will be supplemented by numbers ranging from 001 to 100 for SV 1 and EV 100).
Note:
Some autosamplers may support alphanumerical or graphical setting of the SV or EV parameter. In such case, an arrow will appear in the right side of the given field, and clicking it will open particular Select Vial dialog.
Indicates how many injections will be processed from each vial based on this line in the Sequence Table.
If the Format - Automatically item from Sequence Options dialog has been activated, then, for multiple vials or injections, the File Name pattern will automatically be verified and corrected to ensure that it is in the correct format. In other words, the %v and %i variables will be added to the template.
The sample identification field will be transferred to the headers of all created chromatograms. Maximum number of characters in this field is limited to 64. The parameter corresponds to the Sample ID field from the Single Analysis dialog.
This field serves for information only. After clicking on the cell in this column the 
icon will appear. Using it invokes the list where you can pick from the available variables for composing the name. Variables that can be used are described in the Chromatogram File Name section of the Single Analysis dialog
Sample description - will be transferred to the headers of all created chromatograms. Maximum number of characters in this field is limited to 64. The parameter corresponds to Sample field from the Single Analysis dialog.
This field serves for information only. After clicking on the cell in this column the icon will appear. Using it invokes the list where you can pick from the available variables for composing the name. Variables that can be used are described in the Chromatogram File Name section of the Single Analysis dialog.
Enable to add user comments. Text is editable directly in the cell or after clicking on dropdown arrow of the icon in Comments dialog. Comments column is hidden and empty by default. Sample comments are saved with the chromatogram and can be displayed on Measurement Conditions tab and in Print Preview.
Note:
Comments in the already measured chromatogram are also editable from Measurement Conditions tab.
The value is used in calibration calculations of individual components percentage in the ESTD and ISTD methods. In ISTD enter the sample amount without the addition. Enter values using the same units that were used in the calibration file (Units - Compound parameter in Calibration Options window). The default value is 0. The parameter corresponds to the Amount field of the Single Analysis dialog and the value will be transferred to the chromatogram header.
The amounts of the internal standards for the ISTD method. Other columns for entering amounts of ISTD2 - ISTD10 are hidden by default. This value has to be entered in the same units as those used in the calibration file (as defined in the Units - Compound field of the Calibration Options dialog). The default value is 0. The parameter corresponds to the homonymous field from the Single Analysis dialog and the value will be transferred to the chromatogram header.
This field holds the sample dilution factor - its value is used to multiply each calibrated amount in the Amount column of the Result Table. The default value is 1. The parameter corresponds to Dilution field in the Single Analysis dialog and the value will be transferred to the chromatogram header.
Defines the volume of injection. The response of all compounds in a calibration or when using calibrated calculations is modified by the ratio of this value and Default Injected Volume value from the calibration file. This parameter corresponds to the analogous field from Single Analysis dialog and its value will be transferred to the chromatogram header. The units are set in the Instrument Units dialog (described in the chapter "Right section of System Configuration dialog").
Caution:
When using a directly controlled autosampler this value will define the injection volume! The station then automatically verifies whether the entered volume corresponds to the current autosampler setting.
When the value set in this column is 0, no correction to the Default Injection Volume value will be made. However, when Inj.Vol. of 0 is entered into the sequence when using a directly controlled autosampler, a warning will be given.
AnalysisUserVar1 - AnalysisUserVar3
Sets numerical value of User Variables (up to 3 per row). User Variables can be used for User Column calculations. Columns AnalysisUserVar1 - AnalysisUserVar3 are hidden and set to value 0 by default. Name of the columns can be changed in Setup Columns... through Custom Name line. If the field is left empty, default name AnalysisUserVar1-AnalysisUserVar3 would remain filled in.
Note:
User Variables columns can be edited after sequence end and reprocess in the next batch.
Caution:
Users who do no have the right to Edit Method in the User Accounts dialog cannot edit AnalysisUserVar1 - AnalysisUserVar3; fields will be grayed out.
Defines the chromatogram name pattern. The pattern may use a combination of fixed text and variables (usually entered in the form of % and a character). The same variables may be used as in the Single Analysis dialog, plus %v and %i variables for automatic calculations of vials and injections when measuring multiple analysis from one line.
After clicking on the cell in this column the icon will appear. Using it invokes the list where you can pick from the available variables for composing the file name. You can also enter a subfolder in the chromatogram name. Variables and how to enter the subfolder name are described in the Chromatogram File Name section of the Single Analysis dialog
It is possible to include also the name of a subfolder in the name to store chromatograms into subfolders of the Data Subdirectory. E.g. the MySubProject\MyFileName in the DEMO1 project will result in the MyFilename.prm stored in the C:\CLARITY\Datafiles\DEMO1\DATA\MySubproject folder. If the subfolder doesn't exist, then it will be created when the chromatogram will be stored.
Note:
If variable %n is being used, it will be independent from the variable using the same name in the Single Analysis dialog.
Sets the type of the sample being measured. The possible options are:
Unknown - denotes an unknown sample.
Standard - specifies the calibration standard. Samples marked as Standard must have the calibration level filled in the Lvl column. Chromatograms created on the sequence rows marked as calibration standards will be stored in the calib subdirectory instead of the data subdirectory.
Bypass - this Sample Type type allows for performing an analysis in the Active sequence with controlled autosampler without actually injecting a sample. This may be useful for system clean-up etc..
Blank - in fact a calibration standard with no amount of added sample. The Blank is thus one point of a calibration curve (with the Amount of 0).
Caution:
When measurement of recalibration standard is finished, the calibration file being recalibrated is updated by the sample responses and saved. In case there are any unsaved changes within the calibration file at that moment, those changes are discarded.
Indicates the number of the calibration level when the resulting chromatogram is to be used for automatic recalibration when the Sample Type column of the sample is set to Standard. The file has to be set as the calibration standard in the Sample Type column and a proper calibration file has to be set in the current method as a prerequisite for automatic recalibration.
Indicates the name of the method governing all analyses set on a given line. The active template method name from the
Note:
If you point the mouse cursor to the title of the method, a tooltip will open, in which the title of the method including the entire path will be displayed.
Sets the report style to be used for printing. If no name has been entered, no printing on a given row of the Sequence Table will be performed. After clicking on the cell in this column the icon will appear. Using it opens the Insert File Name dialog for specifying the print method.
Caution:
Print style cannot be selected while a sequence is running.
If you point the mouse cursor to the title of the print style, the yellow tooltip will open, in which the title of the print style including the entire path will be displayed.
The commands Open, Open Calib., Print, Print to PDF, Export Data, Export AIA, Export TXT, Export EZChrom, Export Multidetector, Run Program, Program to Run and Parameters generally correspond to analogous items in the Single Analysis - Post Run Settings tab. All of these columns contain checkboxes, with the exception of the Program to Run column (corresponding to the Program to Run field of the Single Analysis - Post Run Settings tab) and the Parameters column.
Note:
When there is Print, Print to PDF or Export Data set in the Postrun functions, the Open chromatogram Postrun function has to be selected too. Only chromatograms opened in the Chromatogram window can be reported. In sequence with either of the above functions set on a row without Open set as well, sequence row validation fails.
This checkbox allows user to set whether the particular chromatogram will be included in the SST calculations or not.
This checkbox causes the created chromatogram to be opened with the recent stored version of the calibration file, as opposed to the linked calibration when not checked. The checkbox corresponds to the Open with stored calibration checkbox in the right part of the Chromatogram - Results tab.
This checkbox allows to close all chromatograms currently opened in Chromatogram window after the analysis is finished. If the checkbox Open is checked at the same time, the already measured chromatogram will be opened in Chromatogram window. If Open checkbox is not checked, Chromatogram window will remain empty. For multiple injections it works in the same manner. Postrun operations always follow after Close all command.
Note:
To resize a column, point mouse cursor on the edge of the column to display 
, left mouse click and drag to a desired width. All columns, apart from Status, can be resized.
The status bar provides the following information:
State
If the sequence is running, the runtime of the actual sample is displayed. If the sequence is not running, status from the Instrument window in the form of <short status> - <long status> is displayed.
The vial number / injection number from a given vial.
The name of the chromatogram currently being measured.
Active/passive sequence flag.
Chromatogram name control flag showing the settings of the Format section in the Sequence Options dialog