Overlay & Other
Includes commands for working with several chromatograms simultaneously. The maximum number of displayed chromatograms is practically unlimited, the actual limit can be set in the User Options dialog. Most of the commands identified below can be invoked by the icons found in the Overlayand Other toolbars.
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Move | Moves the active chromatogram. |
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Scale | Scales the active chromatogram up or down. |
| Original | Cancels all shift and scaling operations. | |
| 3D View | Displays chromatograms in a three-dimensional perspective. | |
| Clear 3D | Restores the original perspective. | |
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Properties… | Opens the Graph Properties dialog on the Signals tab. |
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Mathematics… | Performs mathematical operations with chromatograms. |
The Overlay mode can be activated using the File - Overlay Mode command, double-clicking on the Overlay inscription in the status bar or clicking on the 
icon. An active OVERLAY mode is indicated by the depressed icon in the Overlay toolbar, highlighted Overlay inscription in the status bar and by the checkmark by the Overlay Mode in the File menu.
Note:
Too many chromatograms opened at once may (especially on slower computers) notably slow down the response of the computer.
In case there is more than one chromatogram (or a chromatogram with more than one signal), it is possible to hide signal by holding CTRL down and clicking on the respective colour in the toolbar. Hidden signals are represented by empty rectangle in the Overlay toolbar (see image below). To once again show the signal, simply hold CTRL down and click on the hidden signal. Alternatively see the Chromatograms topic.
Move
Invoking this command or clicking the Move icon enables you to move the active chromatogram. To use the function, left-click the mouse and hold down while dragging the chromatogram to the new location, then release the button. Exact values of the shift or the possibility to return to the original location are found on the Graph Properties - Signals tab (accessed by using the Chromatogram - Overlay - Properties… command or the Signal Properties icon ). In the shifted chromatogram (signal) all commands from the Chromatogram menu other than those in the Overlay group are disabled and Result Table and peak labels are not shown.
Caution:
The performed moving cannot be stored in the original chromatogram. The only possible way to save such chromatogram is to make a copy of the modified chromatogram by the Overlay - Mathematics - Copy command and save the copy. After finished Move operation, all the values in the Result table will disappear as they are no longer valid.
Note:
Hold Shift while clicking the menu command or icon to apply the move to all signals from the current chromatogram. Hold Ctrl while moving the chromatogram, to limit the moving direction to vertical or horizontal.
Scale
Invoking this command or using the Scale icon allows you to change the scale of the active chromatogram. After invoking the command, left click the mouse and hold it while dragging the chromatogram to the new scale and then release it. The chromatogram will be drawn to the new scale when released. Exact values of the scale operation or the possibility to return to the original state are found on the Graph Properties - Signals tab (accessed by using the Chromatogram - Overlay - Properties… command or the Signal Properties icon ). In the scaled chromatogram (signal) all commands from the Chromatogram menu other than those in the Overlay group are disabled and Result Table and peak labels are not shown. The scaled chromatogram is marked by the MODIFIED inscription in the title bar.
Caution:
The performed scaling operation cannot be stored in the original chromatogram. The only possible way to save such a chromatogram is to make a copy of the modified chromatogram by the Overlay - Mathematics - Copy command and save the copy. After finished Scale operation, all the values in the Result table will disappear as they are no longer valid.
Note:
Hold Shift while clicking the menu command or icon to apply the scaling to all signals from the current chromatogram. Hold Ctrl while moving the chromatogram, to limit the moving direction to vertical or horizontal.
Original
Invoking this command returns the chromatogram to its original location and scale. The initial state is the setting in which the scale is unchanged (Scale = 1) and the chromatogram is not shifted from the start (Offset = 0).
Note:
Hold Shift while clicking the menu command to apply the change to all signals from the current chromatogram.
3D View
Invoking this command enables to display the chromatogram(s) in three-dimensional perspective. After prompting a pair of lines that serve to set the angle and depth of the 3D rendering is shown. Click the mouse button to uniformly space out all displayed chromatograms along the specified line segment. The actual spacing can be modified by changing the X (Y) Offset parameters on the Graph Properties - Signals tab.
Clear 3D
Invoking this command clears the 3D representation mode and restores the original display mode. All chromatograms are restored to their original positions (corresponding to the 0 values of the X (Y) Offset parameters). These values can be set for each chromatogram (signal) separately using the Original button on the Graph Properties - Signals tab.
Properties…
Invoking this command or using the Signal Properties icon allows you to set the explicit shift and scale parameters for the active chromatogram. Invoking the command opens the Signals tab of the Graph Properties dialog (for more details see also chapter Graph Properties - Signals tab)
If any parameter from the Offset & Scale group differs from the default value, the baseline, all commands from the Chromatogram menu other than those in the Overlay group are disabled and Result Table and peak labels are not shown.
Mathematics… (Mathematical Operations dialog)
Invoking this button or using the Mathematics icon enables to perform mathematical operations with chromatograms. After selection, the Mathematical Operations dialog will be displayed.
Mathematical operations are only performed with chromatograms (signals) that are currently open in the Chromatogram window.
Select up to 32 signals in Operand A section in the listbox on the left. The whole chromatogram (all of its signals) will be included when the The whole Chromatogram checkbox has been checked. When unchecked, you may select individual signals (and change their order) from one chromatogram, or select signals from various chromatograms. This is done by simply clicking the row intended for editing and using the arrow icon 
to the right to select the particular chromatogram or signal.
Operand B is selected analogically to the Operand A, if appropriate.
Type of required operation. The following operations are possible:
List of available mathematical operations
Choose method whose parameters (integration table, attached calibration, etc.) will be applied into the created chromatogram. By default, displayed method matches method in the Single Analysis dialog. Leaving this field blank applies default parameters.
The name offered is implicitly derived from the performed mathematical operation - e.g. A-B. To create a custom name, it is necessary to check the Save As Chromatogram checkbox.
If this checkbox is checked, the mathematical result will automatically be saved as a new chromatogram.
If this field is left unchecked, the mathematical result will only be displayed as a new curve, which you may save using the Save or Save As command from the File menu.
In the full version , the resultant chromatogram contains information about the manner of its creation in Chromatogram Audit Trail
