Measurement

Displays the conditions under which the chromatogram was measured. Once selected, the Measurement Conditions tab will be displayed in the lower part of the Chromatogram window, containing Instrument, Event Table and possible Detector sub-tabs; when using a chromatogram that has been measured on an instrument with directly controlled chromatograph, autosampler or any other device having its own tab in the Method Setup dialog, the Sampler, GC, LC Gradient, LC or other sub-tabs are also present. The displayed values are taken from the relevant tabs of the template method describing the way how the chromatogram was measured.

Instrument

This sub-tab is common to all signals in given chromatogram.

The following fields in the Measurement Conditions section are designated for the information purposes only and can be filled in or left blank according to your choice.

Description - brief description of method.

Column - description of the column used.

Mobile Phase - description of mobile phase.

Flow Rate - description of flow rate.

Pressure - description of pressure.

Detection - detection method.

Temperature - description of temperature.

Note - any comment primarily connecting to the method.

Chromatogram - Measurement Conditions

Sample Identification

Fields from the header of the analysis - Single Analysis dialog or the relevant row of the Sequence Table in the Sequence window.

Sample ID

Sample identifier. This corresponds to the Sample ID field from the Single Analysis dialog or the Sample ID column from the Sequence window.

Sample

Description of the sample. This corresponds to the Sample field from the Single Analysis dialog or the Sample column from the Sequence window.

Acquired

Date and time of the end of the measurement. If the date is not accessible (e.g. in some imports), N/A will be displayed.

Analyst

Name of the user who measured the analysis.

Acquisition Parameters

Parameters of measurement common for all signals. The remaining parameters can be found on the Method Setup - Measurement tab.

AutoStop

Notifies whether the Enable Autostop checkbox was used and what was the Run Time value set.

Ext. Start

Notifies whether and by what method the external control of the measurement was defined. This corresponds to the External Start/Stop section of the Method Setup - Measurement tab (see chapter 2.5.2 on page 59 for more details).

Acq. Method

Name of the template method used for measurement.

Range

Specifies the range of the detector signal used. If more than one detector was used to gather data with different Ranges set (including signal units), the <varies> inscription will be displayed here.

Sampling Rate

Specifies the sampling rate of the detector used. If more than one detector was used to gather data with different Sampling Rate set, the <varies> inscription will be displayed here.

Processing Parameters

Parameters of processing method for all signals.

Processing Method

Name, version and date of the method used for processing.

 

Subtraction

Sets the automatic subtraction of selected chromatogram. Subtraction of the chromatogram (e.g. solvent) is indicated by the note Mathematically reprocessed in the printed report. The use of automatic subtraction is also logged into the chromatogram audit trail.

There are two places in Clarity where the subtraction chromatogram can be set - Method Setup - Advanced dialog accessible from the Instrument window and Measurement Conditions - Instrument tab of the Chromatogram window.

If you wish to automatically subtract a chromatogram from the newly measured chromatograms, specify it in the template method in the Method Setup - Advanced dialog.

If you wish to subtract a chromatogram from an already measured chromatogram, specify which on the Chromatogram - Measurement Conditions tab (described further in this chapter).

The subtracted chromatogram is saved into the measured chromatogram, it is not simply linked to it. The changes occurring with the subtraction chromatogram thus do not influence the measured chromatogram from which the background is subtracted. The subtraction, however, does not hold its history - only last subtracted chromatogram's data is saved when it is changed, all older subtraction chromatograms are discarded.

Chromatogram

Field specifying the file with subtraction chromatogram.

Set…

Displays the Open Chromatogram dialog used for selecting the subtracted chromatogram.

None

Cancels subtraction of chromatogram.

Note:

If you only wish to display the chromatogram on the background of the acquisition window without automatic subtraction, use the File - Set Background Chromatogram… command in the Data Acquisition window.

Matching

Selects the subtraction method.

No change - The chromatogram is subtracted from the measured chromatogram without any additional adjustments.

Offset Subtraction Chromatogram - Before subtraction, the Clarity station attempts to “move” the measured chromatogram with the subtracted chromatogram by shifting along the time axis.

Scale Subtraction Chromatogram - Before subtraction, the Clarity station attempts to “align” the measured chromatogram with the subtracted chromatogram by interlining it or shortening the time.

Note:

Alignment of chromatograms is achieved using the biggest peaks in the subtraction chromatogram, i.e. either to the highest peak or to the peak with the largest area.

Chromatogram

Section that contains the additional information on the sample or chromatogram.

Edit…

This button serves for adding some information about the chromatogram or additional info on the sample in the Comments dialog. The button also allows to modify the comment entered prior to the analysis from the Single Analysis or Sequence dialog. This field can then be added to the report setup on the Report Setup - Results tab.

Audit Trail

Using the Show button allows to display the Chromatogram Audit Trail dialog containing the log of events happening with the given chromatogram.

Event Table

This tab shows the setting of the Event Table defined in the method on the Method Setup - Event Table tab. It is common for all signals in case of the multi-detector chromatogram. More information on the Event Table is in the chapter "Event Table".

GLP Info

This tab shows the list of control modules (hardware) configured on the instrument and used for the acquisition of the chromatogram, along with the serial numbers of this hardware. The contents of this tab may be added to the printed reports by checking the GLP Info checkbox on the Report Setup - Method tab.

Detector

The tab displaying detailed measurement parameters. In the case of a multi-detector chromatogram there is a tab for every detector in the configuration of the Instrument.

Sampler

Tab displaying the injection method of the autosampler.

GC/LC/LC Gradient

Tab displaying instrument method of gas or liquid chromatograph.