Linking the calibration to a chromatogram

Analysis Procedure: Step 1 ->Step 2 -> Step 3 -> Step 4

Any chromatogram can be linked to a calibration file, thus automatically providing calibrated results.

• In the Instrument window use the Chromatogram icon to open the Chromatogram window.
• Use the Open Chromatogram icon ① to open chromatogram you want to link the calibration to. We will use the SAMPLE_VIAL_6-1.PRM file, because it is the sample chromatogram to the standards we used to create the calibration.

Upon opening the chromatogram, the data are uncalibrated and no information about the names of individual compounds is available; the peaks in the Result Table are just described according to their retention times. To change this, the appropriate calibration should be linked to these data.

• Select the Results tab ② (it should be opened automatically) and look at the section on the right side of the screen. Use the Set... button ③ in the Calibration File (Peak Table) section to select the calibration file created in the previous section. Any peaks present in the calibration are now identified ④ and have the amounts calculated ⑤.