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GUIDES & DOCUMENTATION

  1. This chapter focuses on processing more chromatograms using Batch dialog. There are 3 main ways of workflow Reprocessing whole sequence, Reprocessing by Method, and Post-run Options. All will be…
  2. Best Practices for Using Clarity Effectively To ensure Clarity operates smoothly and delivers accurate results, it is important to follow certain practices when working with the software and your…
  3. Broad on Narrow Calibration This method first requires the calibration of the column with a series of Narrow Standards and then a calibration with a Broad Standard with known Mn and Mw. It relies on…
  4. Browse through chromatograms from the last folder opened using Open Chromatogram command or browses between selected chromatograms if you selected the "Browse selected chromatograms" option when…
  5. This is a standard procedure used across various chromatography applications - a known amount of a sample is dissolved in a known volume of a solvent and the goal is to determine the percentage…
  6. Calculation Sets the calculation parameters of the method. It links the method to a calibration file, specifies the calculation type, and includes additional settings such as units, response factor…
  7. Calculation This section is located in the right part of the bottom pane of the Chromatogram window. On the left, there is the Result table, or All Signals Result table. The parameters stated here…
  8. Option Clone on first recalibration sets the sequence and calibration to the Safe Calibration Usage mode. This option will create a clone (copy) of calibration defined in the method upon completing…
  9. When using a free calibration, the amounts for each component are calculated using the Response Factor instead of a calibration curve. Create a new calibration file: select File - New or click ⓐ…
  10. The following chapters contain multiple topics which will guide you through the basic principles of using calibrations in Clarity and also introduce you to advanced solutions, for example using the…
  11. Calibration Tabs: Page Setup - Lab. Header - Report Header - Method - Calibration - Chromatogram - Results - Sequence - SST - MS - Audit & Signatures - PDA - NGA - DHA- Lab. Footer Determines…
  12. For each new sequence a calibration file with identical name (*.CAL) is created in the calibration subfolder (CALIB by default) of the current project. This calibration will be based on the…
  13. For the accurate detection of compounds required for gas properties calculations, it is necessary to calibrate the peaks. Create a new calibration or open the nga.cal calibration from the DEMO_NGA…
  14. Calibration Contains commands for managing calibration levels, signals, and compounds, including adding, deleting, clearing, and filtering calibration data. Icon Command Description…
  15. This chapter contains collection of more advanced topics covering calibration usage in Clarity.
  16. The name of the current calibration file is displayed in the window header of the Calibration window. When displaying calibration curves saved in the chromatogram, the header contains the name of…
  17. The Individual Compound tabs contain a table for the displayed compound, several parameter sections, and its calibration curve. Click on any area of the image below to get relevant information…
  18. Calibration Options Tabs: Calibration Options - Defaults The Calibration Options dialog contains various settings that influence calibration. It can be opened via the Calibration - Options... menu…
  19. Calibration Summary Table Error Messages When there are problems calculating the calibration curves for compounds, an error message will appear in the Calibration Summary Table header. The messages…
  20. Analysis Procedure: Step 1 -> Step 2 -> Step 3 -> Step 4 In the main Clarity window, open the Instrument on which you want to test the functions of Sequence. In Clarity Demo it is…
  21. The Clarity CE is an optional Extension for the Clarity Chromatography Station (from version 2.4.4). The Clarity Chromatography Station can acquire data from any CE system with standard analog…
  22. Several terms used in chromatography were substituted by the equivalent terms used in the capillary electrophoresis. Moreover, some of the information fields on the Method Setup - Measurement and…
  23. Open the User Accounts Details window by choosing System - User Details, select the user, and enter the current password. Click on the Change Password button ⓑ and enter and confirm the new password…
  24. Select Chromatogram - Peak - Both or click in the Peak toolbar ⓐ. Set the new position of peak-separating line by clicking in the chromatogram graph ⓑ. You can check, modify the position or…
  25. Check for Updates If your computer is connected to the Internet, Clarity connects to an updating server and checks whether there is a new software update available. When the update program…
  26. The Check Sequence function verifies the sequence before execution. It can be triggered manually from the toolbar or runs automatically when attempting to start a sequence. If any issues are…
  27. The Installation Qualification (IQ) is a procedure confirming that the software has been installed successfully and that correct versions of files are present. Install the Clarity station according…
  28. This chapter serves as a crossroad for the Chromatogram window which is designed to display, adjust and evaluate chromatograms. It can be displayed using the Window - Chromatogram command or using…
  29. Chromatogram A submenu of commands for working with chromatograms. Global Peak Width This command or the icon sets the Peak Width parameter for the whole chromatogram based on the…
  30. Chromatogram Tabs: Page Setup - Lab. Header - Report Header - Method - Calibration - Chromatogram - Results - Sequence - SST - MS - Audit & Signatures - PDA - NGA - DHA- Lab. Footer Sets the…
  31. The chromatogram or chromatograms are displayed in the Graph pane of the Chromatogram window on the Chromatogram tab similarly to the standard Clarity. The window properties including the Overlay…
  32. Chromatogram The Chromatogram window is enhanced by the PDA-related features on the Results and Measurement Conditions tabs.
  33. Chromatogram   Add Signal Adds or replaces the chromatogram signal with a new signal taken as a cut from the PDA data. For more details, see the description below. Display Peak…
  34. The following chapters describe how to edit chromatogram and perform various steps with calibration.
  35. In the Chromatogram - Measurement Conditions pane, Instrument sub-tab, the position in which the chromatogram was acquired is included in the Acquisition and Processing Method info ⓐ. Import…
  36. This chapter focuses mainly on adjusting integration. For proposed general workflow see chapter How to integrate chromatogram Clarity Tips&Tricks videos covering Integration topics can be found…
  37. Chromatogram File Name This field contains a template for creating chromatogram names. Up to 254 characters may be entered including spaces and international characters. The following characters are…
  38. Chromatogram Graph Main graph Displays the chromatogram including the baseline, thus enabling interactive adjustment of the baseline and many other functions. Method of graph display is set by…
  39. The SST tab in the Chromatogram window contains two tables used for setting the validation parameters - Component Table (on the left) and Parameters Table (on the right) of a selected component…
  40. During the chromatogram validation, it is determined whether or not the parameters of individual chromatograms meet the specified criteria. Individual chromatograms can be validated either online (…
  41. Chromatogram window Analysis Procedure: Step 1 -> Step 2 -> Step 3 -> Step 4 The Chromatogram window can be opened also manually by clicking on the Chromatogram icon in the Instrument…
  42. A new DHA tab is available in the Chromatogram window. Furthermore, there is a new DHA menu at the top of the window.
  43. Chromatogram window The Chromatogram window is the central window (opened by clicking the icon) for displaying, modifying and evaluating chromatograms. In the Graph pane, alternatively a…
  44. A new NGA and NGA Summary tabs are available in the Chromatogram window (opened by clicking the icon). Furthermore, there is a new NGA menu at the top of the window.
  45. The Result Table in the EA Extension Chromatogram window has two additional columns performing EA calculations. These columns are: Carbon Amount Ratio Displays the ratio of the carbon amount to the…
  46. Chromatograms Displays the Chromatogram List dialog with a chronological list of all opened chromatograms/signals. Note: Active signal is highlighted in bold letters, hidden signals are displayed…
  47. Connect cables according to one of the diagrams in Fig "Possible types of connection between the Clarity station and a chromatograph". Use a symmetrical connection only if you are sure that the…
  48. The main window of the Clarity station contains Instrument icons as well as a menu for the configuration of the station, opening and closing Instruments etc. Main Clarity window Moving mouse…
  49. Clarity Configuration The following chapter explains how to set the instrument number and type, how to assign Colibrick channels to a specific instrument, and how to name those channels and set…
  50. This chapter describes the basic operating principles for working within the Clarity Dual Chromatogram Mode. Some operations overlap with the standard mode procedures; in such cases, you will be…